Sickle Cell Gene Detection (DNA-Based) Group Size for 8 Gels

Sickle Cell Anemia is a common genetic disease that causes long rods in red blood cells, giving them a "sickled" appearance. These cells get stuck in small capillaries of the blood stream leading to oxygen deprivation that causes pain and organ damage. Sickle Cell Anemia is caused by a single point mutation in the hemoglobin gene that results in a faulty protein. In this experiment, your students will investigate the restriction enzyme that discriminates between HbA (normal) and HbS (disease) genes and perform a simulated test on a patient. Discover how a single nucleotide mutation can profoundly affect a person’s health Explore Mendelian genetics and the molecule methods used to identify sickle cell disease Perform agarose gel electrophoresis to separate differently-sized DNA molecules Load, run, analyze, and size control samples and samples from a family of three to identify mutations in their hemoglobin genesGroup Size:For 8 GelsTime Required:Complete in 45 minutesKit Includes:Instructions, Ready-to-Load QuickStrip DNA Samples, UltraSpec-Agarose, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue DNA Stain.All You Need:DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.Storage:Room Temperature Stable. Storage of Ready-to-Load QuickStrip samples in the Refrigerator is Recommended.

• Kit Includes: Instructions, Ready-to-Load QuickStrip DNA Samples, UltraSpec-Agarose, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue DNA Stain.
• Explore Mendelian genetics and the molecule methods used to identify sickle cell disease
• Perform agarose gel electrophoresis to separate differently-sized DNA molecules
• Load, run, analyze, and size control samples and samples from a family of three to identify mutations in their hemoglobin genes
• Discover how a single nucleotide mutation can profoundly affect a person’s health